Distinction between film loss and enzyme inactivation in protein-film voltammetry: a theoretical study in cyclic staircase voltammetry

Protein-film voltammetry: a theoretical study of the temperature effect using square-wave voltammetry.

Square-wave voltammetry of surface redox reactions is considered as an adequate model for a protein-film voltammetric setup. Here we develop a theoretical approach to analyze the effects of temperature on square-wave voltammograms. The performed simulations address the surface redox reactions featuring slow, modest and fast electron transfer. The theoretical calculations show that the temperatu...

Reactions of complex metalloproteins studied by protein-film voltammetry

The following review explores applications of voltammetric methods for observing reactions of complex metalloproteins. Attention is focused upon the technique of ‘protein-film voltammetry’, in which the protein molecules under investigation are adsorbed on the electrode surface and electrochemically ‘interrogated.’ The experiments address a minuscule sample with high sensitivity, and optimal co...

Cyclic Voltammetry

Thin-film electrochemical sensor electrode for rapid evaluation of electrolytic conductivity, cyclic voltammetry, and temperature measurements

A thin-film electrochemical sensor electrode capable of electrolytic conductivity, cyclic voltammetry, and temperature measurements of electrolyte solutions has been fabricated and characterized. The electrode fabrication and calibration is detailed, showing accuracies of 6 % for electrolytic conductivity measurements over the range of 0.1–100 mS cm 1 and temperature measurement within 2 C from...

Cyclic Voltammetry of Auranofin

The oxidative behavior of Auranofin, 2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranosato- S(triethylphosphine)gold(I), was investigated by using cyclic voltammetry (CV) in 0.1 M Bu(4)NPF(6)/CH(2)Cl(2) and 0.1 M Bu(4)NPF(4)/CH(2)Cl(2) solutions using Pt working and auxiliary electrodes and a Ag/AgCI reference. CV studies at scan rates from 50-2,000 mV/s and Auranofin concentrations between 1 an...